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| Registros recuperados : 36 | |
| 7. |  | MAGALHÃES, M.; MARTINEZ, R. A.; GAIOTTO, F. A. Diversidade genética de Litopenaeus vannamei cultivado na Bahia Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 8, p. 1131-1136, ago. 2007 Título em inglês: Genetic diversity of Litopenaeus vannamei cultivated in Bahia State, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | CIAMPI, A. Y.; GAIOTTO, F. A.; MARQUES, C. M.; WALTER, B. M. T.; GRATTAPAGLIA, D. A. Amplified fragment length polymorphism (AFLP) for population genetics studies of tropical trees. Brazilian Journal of Genetics, Ribeirao Preto, v.19, n.3, p.284, 1996. Supplement.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | MACHADO, F. R.; GAIOTTO, F. A.; SILVA, V. P.; ALMEIDA, T. N. S.; CIAMPI, A. Y. Análise genética populacional de Cedrella fissilis - Cedro (Meliaceae) utilizando marcadores microssatélites. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 61.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | SILVA, A. G.; GAIOTTO, F. A.; CIAMPI, A. Y.; GRATTAPAGLIA, D. Análise de genética populacional de duas espécies de palmito (Euterpe edulis E Euterpe oleracea) utilizando microssatélites. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 5., 2000, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2000. p. 42.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | CUPERTINO, F. B.; LEAL, J. B.; GRATTAPAGLIA, D.; GAIOTTO, F. A. Certificação de paternidade e maternidade em famílias de Eucalyptus spp por meio de marcadores microssatélites. In: CONGRESSO BRASILEIRO DE GENÉTICA, 52., 2006, Foz do Iguaçu, PR. Resumos... Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2006. p. 937.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | BRONDANI, R. P. V.; GAIOTTO, F. A.; MISSIAGGIA, A. A.; KIRST, M.; GRIBELS, R.; GRATTAPAGLIA, D. Microsatellite markers for Ceiba pentandra (Bombacaceae), an endangered tree species of the amazon forest Molecular Ecology Notes, v.3, p.177-179, 2003.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | MISSIAGGIA, A. A.; KIRST, M.; SILVEIRA, F. Q.; GAIOTTO, F. A.; BRONDANI, R. P. V.; GRIBEL, R.; GRATTAPAGLIA, D. Análise da herança de locos microsatélites marcados com fluorescência em sumaúma (Ceiba pentandra) e estimativa de fecundação cruzada em populações naturais. In: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília. Anais: resumos dos trabalhos. Brasília, DF: EMBRAPA-CENARGEN, 1999. p. 67.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | MENEZES, I. P. P. de; HOFFMANN, L. V.; GAIOTTO, F. A.; CIAMPI, A. Y.; BARROSO, P. A. V. Genética populacional de Gossypium mustelinum presentes no Rio de Contas, Bahia, Brasil. In: SEMINÁRIO JOVENS TALENTOS, 6., 2012, Santo Antônio de Goiás. Resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2012. p. 81. (Embrapa Arroz e Feijão. Documentos, 275).| Biblioteca(s): Embrapa Algodão; Embrapa Arroz e Feijão. |
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| 19. | | SILVEIRA, F. Q. de P.; BRONDANI, R. V.; MISSIAGGIA, A. A.; GAIOTTO, F. A.; GRIBEL, R.; COUTINHO, S.; GRATAPAGLIA, D. Desenvolvimento e otimização de locos ssr para estudos de espécies nativas em risco de extinção no Brasil. In: TALENTO Estudantil da Embrapa Recursos Genéticos e Biotecnologia 1998: resumo dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 1998. p. 40. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 34).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 36 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/08/2005 |
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Data da última atualização: |
11/08/2005 |
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Autoria: |
GAIOTTO, F. A.; BRAMUCCI, M.; GRATTAPAGLIA, D. |
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Título: |
Estimation of outcrossing rate in a breeding population of Eucalyptus urophylla with dominant RAPD and AFLP markers. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, v. 95, n. 5/6, p. 842-849, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Eucalyptus breeding is typically conducted by selection in open-pollinated progenies. As mating is controlled only on the female side of the cross, knowledge of outcrossing versus selfing rates is essential for maintaining adequate levels of genetic variability for continuous gains. Outcrossing rate in an open-pollinated breeding population of Eucalyptus urophylla was estimated by two PCR-based dominant marker technologies, RAPD and AFLP, using 11 open-pollinated progeny arrays of 24 individuals. Estimated outcrossing rates indicate predominant outcrossing and suggest maintenance of adequate genetic variability within families. The multilocus outcrossing rate (tm) estimated from RAPD markers (0.93 +or- 0.027), although in the same range, was higher ( alpha > 0.01) than the estimate based on AFLP (0.89 +or- 0.033). Both estimates were of similar magnitude to those estimated for natural populations using isozymes. The estimated Wright's fixation index was lower than expected based on tm possibly resulting from selection against selfed seedlings when sampling plants for the study. An empirical analysis suggests that 18 is the minimum number of dominant marker loci necessary to achieve robust estimates of tm. This study demonstrates the usefulness of dominant markers, both RAPD and AFLP, for estimating the outcrossing rate in breeding and natural populations of forest trees. It is anticipated that there will be an increasing use of such PCR-based technologies in mating-system studies, in view of their high throughput and universality of the reagents, particularly for species where isozyme systems have not yet been optimized. MenosEucalyptus breeding is typically conducted by selection in open-pollinated progenies. As mating is controlled only on the female side of the cross, knowledge of outcrossing versus selfing rates is essential for maintaining adequate levels of genetic variability for continuous gains. Outcrossing rate in an open-pollinated breeding population of Eucalyptus urophylla was estimated by two PCR-based dominant marker technologies, RAPD and AFLP, using 11 open-pollinated progeny arrays of 24 individuals. Estimated outcrossing rates indicate predominant outcrossing and suggest maintenance of adequate genetic variability within families. The multilocus outcrossing rate (tm) estimated from RAPD markers (0.93 +or- 0.027), although in the same range, was higher ( alpha > 0.01) than the estimate based on AFLP (0.89 +or- 0.033). Both estimates were of similar magnitude to those estimated for natural populations using isozymes. The estimated Wright's fixation index was lower than expected based on tm possibly resulting from selection against selfed seedlings when sampling plants for the study. An empirical analysis suggests that 18 is the minimum number of dominant marker loci necessary to achieve robust estimates of tm. This study demonstrates the usefulness of dominant markers, both RAPD and AFLP, for estimating the outcrossing rate in breeding and natural populations of forest trees. It is anticipated that there will be an increasing use of such PCR-based technologies in mating-system st... Mostrar Tudo |
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Palavras-Chave: |
AFLP; RAPD. |
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Thesagro: |
Biotecnologia; Eucalipto. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02217naa a2200193 a 4500
001 1171700
005 2005-08-11
008 1997 bl uuuu u00u1 u #d
100 1 $aGAIOTTO, F. A.
245 $aEstimation of outcrossing rate in a breeding population of Eucalyptus urophylla with dominant RAPD and AFLP markers.
260 $c1997
520 $aEucalyptus breeding is typically conducted by selection in open-pollinated progenies. As mating is controlled only on the female side of the cross, knowledge of outcrossing versus selfing rates is essential for maintaining adequate levels of genetic variability for continuous gains. Outcrossing rate in an open-pollinated breeding population of Eucalyptus urophylla was estimated by two PCR-based dominant marker technologies, RAPD and AFLP, using 11 open-pollinated progeny arrays of 24 individuals. Estimated outcrossing rates indicate predominant outcrossing and suggest maintenance of adequate genetic variability within families. The multilocus outcrossing rate (tm) estimated from RAPD markers (0.93 +or- 0.027), although in the same range, was higher ( alpha > 0.01) than the estimate based on AFLP (0.89 +or- 0.033). Both estimates were of similar magnitude to those estimated for natural populations using isozymes. The estimated Wright's fixation index was lower than expected based on tm possibly resulting from selection against selfed seedlings when sampling plants for the study. An empirical analysis suggests that 18 is the minimum number of dominant marker loci necessary to achieve robust estimates of tm. This study demonstrates the usefulness of dominant markers, both RAPD and AFLP, for estimating the outcrossing rate in breeding and natural populations of forest trees. It is anticipated that there will be an increasing use of such PCR-based technologies in mating-system studies, in view of their high throughput and universality of the reagents, particularly for species where isozyme systems have not yet been optimized.
650 $aBiotecnologia
650 $aEucalipto
653 $aAFLP
653 $aRAPD
700 1 $aBRAMUCCI, M.
700 1 $aGRATTAPAGLIA, D.
773 $tTheoretical and Applied Genetics$gv. 95, n. 5/6, p. 842-849, 1997.
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